Isolation and Characterization of the Tricarboxylate Transporter from Pea Mitochondria
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چکیده
منابع مشابه
Isolation and characterization of the tricarboxylate transporter from pea mitochondria.
The tricarboxylate transporter was solubilized from pea (Pisum sativum) mitochondria with Triton X-114, partially purified over a hydroxylapatite column, and reconstituted in phospholipid vesicles. The proteoliposomes exchanged external [(14)C]citrate for internal citrate or malate but not for preloaded d,l-isocitrate. Similarly, although external malate, succinate, and citrate competed with [(...
متن کاملPurification and characterization of the reconstitutively active tricarboxylate transporter from rat liver mitochondria.
The tricarboxylate transporter has been purified in reconstitutively active form from rat liver mitochondria. The transporter was extracted from mitoplasts with Triton X-114 in the presence of cardiolipin and citrate and was then purified by sequential chromatography on hydroxylapatite, Matrex Gel Orange A, Matrex Gel Blue B, and Affi-Gel 501. Analysis of the purified material via sodium dodecy...
متن کاملIsolation and partial characterization of the glutamate/aspartate transporter from pea leaf mitochondria using a specific monoclonal antibody.
A library of monoclonal antibodies directed against the proteins of the inner mitochondrial membrane was screened for antibodies that could bind to the glutamate/aspartate transporter of pea mitochondria and thereby inhibit its activity. One antibody, 2C7, had the property of inhibiting glutamate and aspartate-dependent oxaloacetate metabolism by pea mitochondria without affecting the metabolis...
متن کاملIsolation and Partial Characterization of the Glutamate/ Aspartate Transporter from Pea Leaf Mitochondria Using a Specific Monoclonal Antibody1
A library of monoclonal antibodies directed against the proteins of the inner mitochondrial membrane was screened for antibodies that could bind to the glutamate/aspartate transporter of pea mitochondria and thereby inhibit its activity. One antibody, 2C7, had the property of inhibiting glutamate and aspartate-dependent oxaloacetate metabolism by pea mitochondria without affecting the metabolis...
متن کاملNAD-Linked Isocitrate Dehydrogenase: Isolation, Purification, and Characterization of the Protein from Pea Mitochondria.
The NAD(+)-dependent isocitrate dehydrogenase from etiolated pea (Pisum sativum L.) mitochondria was purified more than 200-fold by dye-ligand binding on Matrix Gel Blue A and gel filtration on Superose 6. The enzyme was stabilized during purification by the inclusion of 20% glycerol. In crude matrix extracts, the enzyme activity eluted from Superose 6 with apparent molecular masses of 1400 +/-...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.100.4.2030